1965 Australian Championships – Women's singles
| Women's singles | |
|---|---|
| 1965 Australian Championships | |
| Champion | Margaret Smith |
| Runner-up | Maria Bueno |
| Score | 5–7, 6–4, 5–2 ret. |
| Draw | 52 |
| Seeds | 16 |
First-seeded Margaret Smith was the five-time defending champion, and successfully defended her title, defeating Maria Bueno 5–7, 6–4, 5–2 ret. in the final to win the women's singles tennis title at the 1965 Australian Championships.