1964–65 Challenge Cup
| Duration | 5 Rounds |
|---|---|
| Number of teams | 32 |
| Highest attendance | 89,016 |
| Broadcast partners | BBC 1 |
| Winners | Wigan |
| Runners-up | Hunslet |
| Lance Todd Trophy | Ray Ashby Brian Gabbitas (shared) |
The 1964–65 Challenge Cup was the 64th staging of rugby league's oldest knockout competition, the Challenge Cup.
The final was contested by Wigan and Hunslet at Wembley Stadium. Wigan won the match 20–16, with Wigan's Ray Ashby and Hunslet's Brian Gabbitas jointly receiving the Lance Todd Trophy.