1965 U.S. National Championships – Women's singles
| Women's singles | |
|---|---|
| 1965 U.S. National Championships | |
| Champion | Margaret Smith |
| Runner-up | Billie Jean Moffitt |
| Score | 8–6, 7–5 |
| Seeds | 8 |
First-seeded Margaret Smith defeated fifth-seeded Billie Jean Moffitt 8–6, 7–5 in the final to win the women's singles tennis title at the 1965 U.S. National Championships.