2000–01 Czech First League
| Season | 2000–01 |
|---|---|
| Champions | Sparta Prague |
| Relegated | České Budějovice Viktoria Plzeň |
| Champions League | Sparta Prague Slavia Prague |
| UEFA Cup | Sigma Olomouc Marila Příbram Viktoria Žižkov Slovan Liberec |
| Intertoto Cup | Blšany Synot |
| Matches played | 240 |
| Goals scored | 613 (2.55 per match) |
| Top goalscorer | Vítězslav Tuma (15) |
| Biggest home win | Synot 5–0 Plzeň |
| Biggest away win | Ostrava 1–5 Sparta Prague Olomouc 1–5 Slavia Prague |
| Highest scoring | Sparta Prague 4–4 Slavia Prague |
| Highest attendance | 16,350 Sparta Prague 4–4 Slavia Prague |
| Lowest attendance | 457 Žižkov 3–3 Plzeň |
| Average attendance | 4,549 |
2001–02 → | |
The 2000–01 Czech First League, known as the Gambrinus liga for sponsorship reasons, was the eighth season of top-tier football in the Czech Republic.