2000–01 Slovak Cup
| Tournament details | |
|---|---|
| Country | Slovakia | 
| Teams | 42 | 
| Final positions | |
| Champions | Inter Bratislava | 
| Runners-up | SCP Ružomberok | 
The 2000–01 Slovak Cup was the 32nd season of Slovakia's annual knock-out cup competition and the eighth since the independence of Slovakia. It began on 25 July 2000 with the matches of preliminary round and ended on 8 May 2001 with the final. The winners of the competition earned a place in the qualifying round of the UEFA Cup. Inter Bratislava were the defending champions.