2000 Italian Open – Women's singles

Women's singles
2000 Italian Open
Champion Monica Seles
Runner-up Amélie Mauresmo
Score6–2, 7–6(7–4)
Draw56
Seeds16

Monica Seles defeated Amélie Mauresmo in the final, 6–2, 7–6(7–4) to win the women's singles tennis title at the 2000 Italian Open. It was her third title of the year, and the 47th of her career.

Venus Williams was the defending champion, but lost in the third round to Jelena Dokic.

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