2016 One-Day Cup
| Dates | 5 June – 17 September 2016 |
|---|---|
| Administrator(s) | England and Wales Cricket Board |
| Cricket format | Limited overs cricket (50 overs) |
| Tournament format(s) | Group stage and knockout |
| Champions | Warwickshire Bears |
| Participants | 18 |
| Matches | 79 |
| Most runs | Sam Hain (540) |
| Most wickets | Matt Coles (24) |
| Official website | ecb.co.uk |
The 2016 Royal London One-Day Cup tournament was a limited overs cricket competition that formed part of the 2016 domestic cricket season in England and Wales. Matches were contested over 50 overs per side and have List A status. All eighteen First-class counties competed in the tournament. Defending champions Gloucestershire went out in the group stage, as Warwickshire Bears beat Surrey in the final.