2024 National Bank Open – Women's singles

Defending champion Jessica Pegula defeated Amanda Anisimova in the final, 6–3, 2–6, 6–1 to win the women's singles tennis title at the 2024 Canadian Open. It was her third WTA 1000 and sixth career WTA Tour title. Pegula became the first player to defend the women's singles title since Martina Hingis in 2000. Ranked No. 132, Anisimova became the lowest-ranked women's singles finalist at the Canadian Open since Alycia Moulton in 1984.

This marked the second WTA 1000 tournament to have five quarterfinalists from the same country, the United States, and the first such instance since the 2009 China Open when the WTA 1000 format was first introduced and the most American quarterfinalists on the WTA Tour since 2001. For the first time since 2001, two American women contested the final.