2025 Australian Open – Wheelchair women's singles

Yui Kamiji defeated Aniek van Koot in the final, 6–2, 6–2 to win the women's singles wheelchair tennis title at the 2025 Australian Open. It was her third Australian Open singles title and ninth major singles title overall.

Diede de Groot was the four-time reigning champion, but did not participate this year due to a hip injury. This marked the first major since the 2020 French Open not to be won by de Groot, a 15-major win streak.