2025 Australian Open – Women's singles

Madison Keys defeated two-time defending champion Aryna Sabalenka in the final, 6–3, 2–6, 7–5 to win the women's singles tennis title at the 2025 Australian Open. It was her first major title. Keys saved a match point en route to the title, in the semifinals against Iga Świątek. She was the fourth woman in the Open Era (after Evonne Goolagong Cawley at the 1980 Wimbledon Championships, Mary Pierce at the 1995 Australian Open and Li Na at the 2011 French Open) to defeat four top-10 seeds en route to a major title.

Sabalenka was attempting to become the first woman to win three consecutive Australian Open singles titles since Martina Hingis in 1999. She retained the world No. 1 singles ranking after Świątek lost in the semifinals. Coco Gauff was also in contention for the top ranking at the beginning of the tournament.

Eva Lys was the first lucky loser to reach the fourth round of the women's singles event in the Open Era, and only the sixth to do so at any major in the Open Era.