| ALCO RS-27 | 
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| Minnesota Commercial Railway 318 at Minneapolis, July 2009 | 
| | Type and origin | 
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 | Power type | Diesel-electric | 
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 | Builder | ALCO | 
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 | Model | RS-27 | 
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 | Build date | December 1959 – October 1962 | 
|---|
 | Total produced | 27 | 
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The ALCO RS-27 (specification DL-640) was a diesel-electric locomotive built by ALCO between December 1959 and October 1962.
Only 27 examples were manufactured. With ALCO's introduction of the Century Series line in 1963, the C-424 (specification DL-640A) replaced the RS-27 in the builder's catalog.  Today, the Minnesota Commercial Railway has the only two RS-27s left in existence; however both have been placed into storage.