Ayreon Universe – The Best of Ayreon Live, alternatively known as Ayreon Universe – Best of Ayreon Live or simply Ayreon Universe, is a live album and DVD/Blu-ray by Arjen Anthony Lucassen's progressive rock/metal rock opera project Ayreon, released on 30 March 2018. Unlike Ayreon's first live album The Theater Equation, which was a stage performance of the album The Human Equation, Ayreon Universe was performed as a traditional concert, with two additional songs from Star One, another project by Lucassen. It was recorded during a series of concerts in September 2017, which were the first ever official Ayreon concerts, although The Theater Equation was released (but not performed) as an official Ayreon product.
The performance featured 16 singers, all of whom had previously worked together with Lucassen on his studio albums.