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Highway 38 highlighted in red  | 
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| Maintained by City of Kingston, South Frontenac and Central Frontenac Township | 
| Length | 66.9 km (41.6 mi) | 
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| Existed | April 25, 1934–January 1, 1998 | 
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| South end |  Highway 401 in Kingston | 
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| North end |  Highway 7 near Sharbot Lake | 
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| Country | Canada | 
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| Province | Ontario | 
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| Counties | Frontenac | 
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| Major cities | Kingston | 
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| Towns | South Frontenac Township, Central Frontenac Township | 
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| Villages | Hartington, Harrowsmith, Verona, Godfrey, Parham, Tichborne, Sharbot Lake | 
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King's Highway 38, commonly referred to as Highway 38, was a provincially maintained highway in the Canadian province of Ontario. The 66.9-kilometre (41.6 mi) road connected Highway 2 and Highway 401 in Kingston with Highway 7 west of Perth. It was designated in 1934 and remained relatively unchanged throughout its existence, aside from some minor diversions and a rerouting through Kingston as a result of the construction of Highway 401 in the mid-1950s. At the beginning of 1998, the entire highway was transferred to the municipalities of Frontenac County through which it travelled: Kingston, South Frontenac and Central Frontenac. Today the former highway is named Road 38 and Gardiners Road, but is still referred to as Highway 38 by locals.