John Fleming, 2nd Lord Fleming

John Fleming, 2nd Lord Fleming (c. 1465 – 1 November 1524) was a Scottish nobleman.

He was the son of Malcolm Fleming, 1st Lord Fleming, and Euphame Livingstone, a daughter of James Livingston, 1st Lord Livingston. In 1514, he was one of the lords charged with the care of the infant James V and his younger brother, Alexander Stewart, Duke of Ross. In the spring of 1522 he and Lord Hay, the king's secretary, visited France on a diplomatic mission.

He was assassinated by John Tweedie of Drumelzier, and his followers, while hawking.

His son was Malcolm Fleming, 3rd Lord Fleming.