Jalan Bukit Gambir
| Johor Route 33 | |
|---|---|
| Major junctions | |
| Northeast end | Sungai Mati | 
|  FT 23 Federal Route 23 J137 Jalan Panchor J34 Jalan Bukit Serampang  | |
| Southwest end | Bukit Serampang | 
| Location | |
| Country | Malaysia | 
| Primary destinations  | Serom, Gerisek, Panchor, Bukit Kepong | 
| Highway system | |
Jalan Bukit Gambir (Johor state route J33) is a major road in Johor, Malaysia. The roads is also a main route to North–South Expressway Southern Route via Bukit Gambir Interchange.