Phosphatidate phosphatase
| Phosphatidate phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.3.4 | ||||||||
| CAS no. | 9025-77-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme phosphatidate phosphatase (PAP, EC 3.1.3.4) is a key regulatory enzyme in lipid metabolism, catalyzing the conversion of phosphatidate to diacylglycerol:
- a 1,2-diacylglycerol 3-phosphate + H2O a 1,2-diacyl-sn-glycerol + phosphate
The reverse conversion is catalyzed by the enzyme diacylglycerol kinase, which replaces the hydroxyl group on diacylgylcerol with a phosphate from ATP, generating ADP in the process.
In yeast, the forward direction is Mg2+-dependent, while the reverse process is Ca2+-dependent. PAP1, a cytosolic phosphatidate phosphatase found in the lung, is also Mg2+-dependent, but PAP2, a six-transmembrane-domain integral protein found in the plasma membrane, is not.