Plant transformation vector

Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants. The most commonly used plant transformation vectors are T-DNA binary vectors and are often replicated in both E. coli, a common lab bacterium, and Agrobacterium tumefaciens, a plant-virulent bacterium used to insert the recombinant DNA into plants.

Plant transformation vectors contain three key elements:

  • Plasmids Selection (creating a custom circular strand of DNA)
  • Plasmids Replication (so that it can be easily worked with)
  • Transfer DNA (T-DNA) region (inserting the DNA into the agrobacteria)
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