Rhodamine B

Rhodamine B
Names
	Preferred IUPAC name 9-(2-Carboxyphenyl)-6-(diethylamino)-N,N-diethyl-3H-xanthen-3-iminium chloride
	Other names Rhodamine 610, C.I. Pigment Violet 1, Basic Violet 10, C.I. 45170
Identifiers
CAS Number	81-88-9 ;
3D model (JSmol)	Interactive image;
ChEBI	CHEBI:52334 ;
ChEMBL	ChEMBL428971 ;
ChemSpider	6439 ;
ECHA InfoCard	100.001.259
KEGG	C19517 ;
PubChem CID	6694;
UNII	K7G5SCF8IL ;
CompTox Dashboard (EPA)	DTXSID6042369 ;
	InChI InChI=1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H Key: PYWVYCXTNDRMGF-UHFFFAOYSA-N ;
	SMILES CCN(CC)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](CC)CC)C=C3O2)C4=CC=CC=C4C(=O)O.[Cl-];
Properties
Chemical formula	C28H31ClN2O3
Molar mass	479.02
Appearance	Green powder
Melting point	210 to 211 °C (410 to 412 °F; 483 to 484 K) (Decomposes)
Solubility in water	8 to 15 g/L (20 °C)
Hazards
Safety data sheet (SDS)	MSDS
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). verify (what is ?) Infobox references

Rhodamine B /ˈroʊdəmiːn/ is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers.

Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium. Rhodamine dyes are also used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.