U.S. Route 27 in Tennessee
| US 27 highlighted in red | |||||||
| Route information | |||||||
| Maintained by TDOT | |||||||
| Length | 144.8 mi (233.0 km) | ||||||
| Existed | 1926–present | ||||||
| Tourist routes | Cumberland Historic Byway | ||||||
| Major junctions | |||||||
| South end | US 27 at the Georgia state line | ||||||
| North end | US 27 at the Kentucky state line | ||||||
| Location | |||||||
| Country | United States | ||||||
| State | Tennessee | ||||||
| Counties | Hamilton, Rhea, Roane, Morgan, Scott | ||||||
| Highway system | |||||||
| 
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U.S. Route 27 (US 27) in Tennessee runs from the Georgia state line in Chattanooga to the Kentucky state line in Isham. It forms the informal border between the Eastern and Central timezones of the state. It stays, however, in the Eastern Time Zone.