2016 Czech Senate election|
|
| 
|  | First party | Second party | Third party |  
|  |  |  |  |  
| Leader | Pavel Bělobrádek | Petr Fiala | Andrej Babiš |  
| Party | Lidovci | ODS | ANO |  
| Seats won | 6 | 3 | 3 |  
| First round | 74,709 8.48%
 | 103,216 11.71%
 | 151,388 17.18%
 |  
| Second round | 78,448 18.50%
 | 42,551 10.03%
 | 92,051 21.71%
 |  
| 
 |  
|  | Fourth party | Fifth party | Sixth party |  
|  |  |  |  |  
| Leader | Bohuslav Sobotka | Miroslav Kalousek | Petr Gazdík |  
| Party | ČSSD | TOP 09 | STAN |  
| Seats won | 2 | 2 | 2 |  
| First round | 128,830 14.62%
 | 70,653 8.02%
 | 43,234 4.91%
 |  
| Second round | 55,622 13.12%
 | 30,820 7.27%
 | 25,389 5.99%
 |  |
 | 
Senate elections were held in the Czech Republic on 7 and 8 October 2016, with a second round on 14 and 15 of October. The first round was held alongside regional elections and several municipal referendums, notably in a referendum in Brno on the location of the town's train station.
The governing coalition of the Czech Social Democratic Party (ČSSD), ANO 2011 and the Christian and Democratic Union (KDU-ČSL) retained a majority in the full Senate, with the KDU-ČSL making the largest gains, while the Social Democrats incurred the greatest losses, retaining only two of their twelve seats up for re-election. ANO 2011 won the first round with fourteen candidates advancing, but only three of them were elected, which was a disappointment to the party. The most successful opposition party was the centre-right Civic Democratic Party. It had 6 candidates advancing and four of them elected (including Zdeněk Nytra who ran as independent).