Ontario Highway 53

King's Highway 53, commonly referred to as Highway 53, was a provincially maintained highway in the southern portion of the Canadian province of Ontario that connected Woodstock to Hamilton via Brantford. The 76.5-kilometre (47.5 mi) route served as a southerly bypass to Highway 2, avoiding Paris, Ancaster and Hamilton. In addition to the previously mentioned cities, Highway 53 served the communities of Cathcart and Burford.

First designated in 1935, the route remained in place until it was downloaded to local jurisdiction in 1997. For much of its history, the road was concurrent with Highway 2 between Woodstock and Eastwood, as well as between Brantford and Ancaster. Today the route is known by various local names, including Oxford County Road 55, Brant County Highway 53, Wilson Street, Garner Road and Rymal Road. It serves as an alternative to Highway 2 or Highway 403.